Fig. 6

Combination therapy of hCMSCs and liraglutide attenuated ALI at 2 days in vivo. H&E staining (a). The pathological sections were imaged using a × 20 objective; 10 fields were randomly selected for scoring and the lung injury index was calculated according to the formula (b). Wet-to dry-ratio (W/D) (c). Neutrophils, leukocytes, and macrophages in mouse bronchoalveolar lavage fluid (BALF) (d–f) were counted under eight randomly selected fields using a microscope of × 10 magnification. ELISA assay (g–j) was performed to detect the secretion of many cytokines such as TNF-α, IL-1β, IL-6, and IL-10 in BALF. The data for each histogram is presented by mean ± SD. Significant differences between two groups were expressed as **P < 0.01, *P < 0.05, n = 6 per group. Data are represented as mean ± SD