Fig. 2

Effects of DH and LPA on MSC proliferation and migration. A scratch was made using a P1000 pipette tip and cultured in the presence of DH or LPA for 7 days. The distance between edges was monitored under a microscope to determine the proliferation rate upon treatment (a). Representative pictures of cells at the bottom side of the inserted chamber after 6-h incubation stained with Hoechst-33342 (b). The number of migrated cells was counted and reported as mean ± SEM; n = 3, *P < 0.05, ***P < 0.001, Student’s t test (c). MSC numbers were enumerated after treatment with DH and LPA with trypan blue exclusion (d). Statistical analysis for live cells is shown as mean ± SEM; n = 3, *P < 0.05, ***P < 0.001, Student’s t test. Numbers of dead cells were not significantly different among treatments