Fig. 5
From: Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile
Endometrial stromal cells (eSCs) modulate the proliferation, activation, and differentiation status of CD4+ T cells. a eSCs suppressed the proliferation of CD4+ T cells in direct contact and transwell culture systems. Box and whisker plots showing the minimum and maximum distribution of the proliferation index of CD4+ T cells. b Scatter plot showing suppression of activation (CD25+) in CD4+ T cells by eSCs in transwell cultures (mean ± standard deviation of the mean [SD]). c, d Representative gating strategy to determine CD3+CD4+ T cells and their differentiation status based on expression of CD27 and CD45RA. e Scatter plot showing the significant drop in CD27+CD45RA- central memory (CM) CD4+ T cells with eSC exposure in transwell culture (mean ± SD). f Scatter plot showing no effect on the relative percentage of CD27+CD45RA+ naive CD4+ T cells with eSC exposure (mean ± SD). g Scatter plot demonstrating the significant increase in relative percentages of CD27-CD45RA- effector memory (EM) CD4+ T cell with eSC exposure in both direct contact and transwell cultures (mean ± SD). h Scatter plot showing no effect on the relative percentage of CD27-CD45RA+ effector memory T cells re-expressing CD45RA (TEMRA) CD4+ T cells with eSC exposure (mean ± SD). (n = 12) *P < 0.05; **P < 0.01; ****P < 0.0001