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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction

Fig. 3

Role of P2X7 in EMF-enhanced osteogenic differentiation in h-MSCs. a qRT-PCR analysis of osteogenic markers (RUNX2, ALP, and OPN) expression in h-MSCs cultured in osteogenic medium, osteogenic medium+5 μM A740003, osteogenic medium+15 Hz/1 mT EMF, and osteogenic medium+15 Hz/1 mT EMFs+5 μM A740003 for 7 days. The mRNA expression of RUNX2, ALP, and OPN was normalized against GAPDH. b Western blot analysis of osteogenic marker (RUNX2 and OPN) expression in h-MSCs cultured in osteogenic medium, osteogenic medium+5 μM A740003, osteogenic medium+15 Hz/1 mT EMF, and osteogenic medium+15 Hz/1 mT EMFs + 5 μM A740003, respectively, for 7 days. The protein expression of RUNX2 and OPN normalized against GAPDH. c Alizarin red S staining of h-MSCs treated with osteogenic medium, osteogenic medium + 5 μM A740003, osteogenic medium + 15 Hz/1 mT EMFs, and osteogenic medium + 15 Hz/1 mT EMFs + 5 μM A740003 for 7 days. Data represent means ± SD. Statistically significant differences are indicated; n = 3; *P < 0.05, vs. control

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Stem Cell Research & Therapy

ISSN: 1757-6512