Table 1 Sequences of primers used in qPCR
Gene | Primer | Sequence 5′–3′ | Amplicon length (bp) | Accession no. |
|---|---|---|---|---|
Vimentin | F: | GCAGGATTTCTCTGCCTCTT | 203 | XM_014846038.1 |
R: | TATTGCTGCACCAAGTGTGT | |||
Decorin | F: | GATGCAGCTAGCCTGAGAGG | 248 | XM 014841263.1 |
R: | GTGTTGTATCCAGGTGGGCA | |||
Col2A1 | F: | ATTCCTGGAGCCAAAGGATCTGCT | 148 | XM_014850051.1 |
R: | TGAAGCCAGCAATACCAGGTTCAC | |||
ACAN | F: | TGGTGTCCTCTTCTTGTCGCTTTC | 160 | XM_014733894.1 |
R: | ACGATACATTTGCTGTGCTTCGGC | |||
COMP | F: | AGTGTCGCAAGGATAACTGCGTGA | 238 | NM_001081856.1 |
R: | TCCTGATCTGTGTCCTTCTGGTCA | |||
SOX-9 | F: | GAACGCCTTCATGGTGTGGG | 225 | XM_014736619.1 |
R: | TTCTTCACCGACTTCCTCCG | |||
Runx-2 | F: | CCAAGTGGCAAGGTTCAACG | 165 | XM_014837484.1 |
R: | TGTCTGTGCCTTCTGGGTTC | |||
ALP | F: | AGGCCAAGATGAGGGTAGCA | 352 | XM_014852743.1 |
R: | TCTCTGGCACTAAGGAGTTGGT | |||
VEGF | F: | CCCACTGCGGAGTTCAACAT | 167 | XM_014837457.1 |
R: | TTTCTCCGCTCTGAGCAAGG | |||
CD44 | F: | CCTCCAGCGAAAGAAGCACT | 137 | NM_001085435.2 |
R: | GGCTCGGTCTTTGGTAGTGG | |||
p53 | F: | TACTCCCCTGCCCTCAACAA | 252 | U37120.1 |
R: | AGGAATCAGGGCCTTGAGGA | |||
Bax | F: | TTCCGACGGCAACTTCAACT | 204 | XM_005596728.1 |
R: | GGTGACCCAAAGTCGGAGAG | |||
Bcl-2 | F: | TTCTTTGAGTTCGGTGGGGT | 164 | XM_014843802.1 |
R: | GGGCCGTACAGTTCCACAA | |||
p21 | F: | GAAGAGAAACCCCCAGCTCC | 241 | XM_003365840.2 |
R: | TGACTGCATCAAACCCCACA | |||
Sod1 (Cu/Zn SOD) | F: | CATTCCATCATTGGCCGCAC | 130 | NW_001867397.1 |
R: | GAGCGATCCCAATCACACCA | |||
Sod2 (Mn SOD) | F: | GGACAAACCTGAGCCCCAAT | 125 | NW_001867408.1 |
R: | TTGGACACCAGCCGATACAG | |||
CAT | F: | ACCAAGGTTTGGCCTCACAA | 112 | XM_014851065.1 |
R: | TTGGGTCAAAGGCCAACTGT | |||
GAPDH | F: | GATGCCCCAATGTTTGTGA | 250 | NM_001163856.1 |
R: | AAGCAGGGATGATGTTCTGG |