Fig. 2
From: Xenofree generation of limbal stem cells for ocular surface advanced cell therapy
Expression of stemness and differentiation markers in LSC grown in XSHEM and CnT07. a Analysis of the mRNA expression levels for corneal differentiation markers CK3 and CK12. Their expression was minimal when compared with CO, although LSC cultured in CnT07 expressed more CK3 than those cultured in XSHEM. b Analysis of the mRNA expression levels for the stemness marker ΔNp63α did not show differences between LSC cultured in XSHEM or CnT07. c Analysis of the mRNA expression levels for Bmi1, ABCG2, CK19, CK15, and PAX6. d Immunofluorescence for p63, CK3, and CK12. Cultures showed a broad positivity for p63, while were widely negative for CK3 and CK12. Results are presented as mean ± SE from three independent experiments. Statistical analysis was performed using two-tailed Student’s t tests (*p < 0.05; ***p < 0.001; n.s, not significant). CK, cytokeratin; CO, human corneal epithelial cells; XSHEM, xenofree supplemented hormonal medium. Bar = 10 μm for p63. Bar = 25 μm for CK3 and CK12