Fig. 4
From: Xenofree generation of limbal stem cells for ocular surface advanced cell therapy
Expression of IL-6 and SDF-1. a ELISA assay detected increased expression of human IL-6 during the co-cultures of LSC with PLA. PLA monocultures also secreted higher levels of IL-6 during the first day of seeding. b Analysis of mRNA expression levels of IL-6 in 3T3 and PLA monocultures, and in LSC co-cultures with both feeder layers. c Analysis of mRNA expression levels of SDF-1 in 3T3 and PLA monocultures, and in LSC co-cultures with both feeder layers. Statistical analysis was performed using two-tailed Student’s t tests (**p < 0.01 compared to LSC-3T3; ***p < 0.001 compared to LSC-3T3; &p < 0.001 compared to PLA; %p < 0.01 compared to 3T3). D, day; IL-6, interleukin-6; LSC, limbal stem cells; LSC-3T3, co-cultures of LSC with 3T3 feeder layers; LSC-PLA, co-cultures of LSC with PLA feeder layers; PLA, processed lipoaspirate cells; SDF-1, stromal derived factor