Fig. 2

Cytoviability and metabolic characteristics of the gelMA overlay hBM-MPC micromass cultures during TGFβ3-induced chondrogenesis. a Oblique bright-field and epifluorescence imaging of micromass cultures on days 7, 10, and 14. Under oblique bright-field microscopy, the matrix rich core of the culture appeared as a yellow field. Under epifluorescence microscopy, Live/Dead™ staining of the same cultures revealed that most of the cells were alive (Calcein: green), with very few dead cells present (Et-HD: red). Scale bar = 1 mm. b MTS assay. No significant changes in cell proliferation were seen over the 14-day culture period. c Medium glucose concentration analysis. The results showed that glucose consumption by the micromass cultures increased over culture time