Fig. 1
From: PAMAM-cRGD mediating efficient siRNA delivery to spermatogonial stem cells
Physicochemical characterization of nano-siRNA complexes. a TEM images of G5 and the complexes of G5-siRNA (dendriplexes) and G5-cRGD-siRNA (cRGD-dendriplexes) at the N/P ratio of 10. Scale bar is 100 nm. Particle size distribution of nano-siRNA complexes obtained by TEM images. Fifty nanoparticles were counted. b Representative images of gel retardation assay for G5-siRNA and G5-cRGD-siRNA at different N/P ratios. Lane 1: naked siRNA, lanes 2–8: nano-siRNA complexes (N/P ratios 2.5:1, 5:1, 7.5:1, 10:1, 20:1, 30:1 and 40:1). c Zeta potentials of nano-siRNA complexes at different N/P ratio