Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Long non-coding RNA FER1L4 promotes osteogenic differentiation of human periodontal ligament stromal cells via miR-874-3p and vascular endothelial growth factor A

Fig. 5

miR-874-3p inhibited the osteogenic differentiation of PDLSCs and reversed the effects of FER1L4. a Images of ALP staining on day 7 of osteogenic differentiation and Alizarin red S staining on day 14 of osteogenic differentiation in PDLSCs transfected with miR-NC or miR-874-3p mimic. Scale bar, 100 μm. Histograms show ALP activity and quantification of Alizarin red S staining by spectrophotometry. b Transfection efficiency of miR-874-3p overexpression in PDLSCs and relative mRNA expression of ALP, RUNX2, and OCN on day 7 of osteogenic induction. c Western blot analyses of RUNX2 and OCN on day 7 of osteogenic induction in PDLSCs transfected with miR-NC or miR-874-3p mimic. Histograms show quantification of the band intensities. d Images of ALP staining and Alizarin red S staining in PDLSCs co-transfected with pQLL-FER1L4 with or without miR-874-3p. Results are presented as mean ± SD (*p < 0.05, **p < 0.01)

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512