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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: LncRNA KLF3-AS1 in human mesenchymal stem cell-derived exosomes ameliorates pyroptosis of cardiomyocytes and myocardial infarction through miR-138-5p/Sirt1 axis

Fig. 1

Identification of human mesenchymal stem cells and exosomes derived from human mesenchymal stem cells. Note: The biomarkers for HMSCs were measured including CD34, CD14, CD45, CD29, CD44, and CD106 (a). HMSCs, human mesenchymal stem cells. TEM demonstrated that the exosomes secreted by HMSCs were round vesicles (bars = 200 nm) (b). The detection on exosome-specific biomarker by FCM showed exosomes had positive expressions of TSG101 and CD63 (c). Western blot also detected the protein expressions of TSG101 and CD63 (d). Observation under a fluorescent microscope showed that PKH67-labeled exosomes were in green,α-SMA in red, and the cardiomyocyte nucleus DNA labeled by DAPI were in blue (× 400) (e). The ingestion of exosomes was measured by FCM (f). HMSCs, human mesenchymal stem cells; FCM, flow cytometry; TEM, transmission electron microscope

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Stem Cell Research & Therapy

ISSN: 1757-6512