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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: LncRNA KLF3-AS1 in human mesenchymal stem cell-derived exosomes ameliorates pyroptosis of cardiomyocytes and myocardial infarction through miR-138-5p/Sirt1 axis

Fig. 5

KLF3-AS1 can bind with miR-138-5p to regulate expression of Sirt1. Note: After cardiomyocytes were transfected with miR-138-5p inhibitor, the expressions of miR-138-5p (a), KLF3-AS1 (b), and Sirt1 (c, d) were determined by qRT-PCR and Western blot. Then, cardiomyocytes transfected with KLF3-AS1 overexpression vector were measured for expressions of miR-138-5p (f), KLF3-AS1 (e), and Sirt1 (g, h). The predicted bind sites of miR-138-5p with KLF3-AS1 and Sirt1 are listed in i. Dual dual-luciferase reporter assay confirmed the binding of miR-138-5p with KLF3-AS1 (j) and with Sirt1 (k). *P < 0.05, **P < 0.01, vs control group or mimic+mut-KLF3-AS1 group or mimic+mut-Sirt1 group; qRT-PCR, quantitative reverse transcription-polymerase chain reaction

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Stem Cell Research & Therapy

ISSN: 1757-6512