Fig. 6

HLA-ABC is expressed at the plasma membrane of IFNγ + IL-1β- and IFNα-treated iPSC-derived pancreatic endocrine cells. Control iPSCs (HEL115.6) were differentiated into pancreatic endocrine cells and exposed to IFNγ + IL-1β or IFNα for 24 h. Cells were dissociated, and expression of insulin (Ins), glucagon (Gcg), and HLA-ABC was evaluated by flow cytometry. a Four cell populations were identified using Ins (indicated as I+) and Gcg (indicated as G+) expression (respectively I+G−, I−G+, I+G+, and I−G−). b For each cell population defined above, HLA-ABC expression was plotted against the forward scatter signal. c A histogram showing the percentages of cells expressing HLA-ABC at their surface, for each of the cell population identified (n = 4 independent experiments). **p ≤ 0.01, ***p ≤ 0.001 (one-way ANOVA followed by paired Student’s t test; significant compared to corresponding I−G− cell population); ^$p ≤ 0.05, ^$$p ≤ 0.01, ^$$$p ≤ 0.0001 (one-way ANOVA followed by paired Student’s t test; significant compared to respective control condition)