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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Long noncoding RNA HULC accelerates the growth of human liver cancer stem cells by upregulating CyclinD1 through miR675-PKM2 pathway via autophagy

Fig. 3

HULC increases miR675. a RNA immunoprecipitation (RIP) with anti-METTL3 followed by RT-PCR. IgG RIP served as the negative control. b Real-time RNA Immunoprecipitation (RIP) with anti-METTL3 followed by RT-PCR. IgG RIP served as the negative control. c RNA Immunoprecipitation (RIP) with anti-m6A followed by RT-PCR with Pri-miR675 promoter primers. IgG RIP served as the negative control. d Northern blotting with Biotin-miR675 probe. U6 served as the internal control. e Real-time RT-PCR with miR675 primers. U6 served as an internal control

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