Fig. 4

HULC enhances the interaction between LC3 and Sirt1. a The bioinformatics analysis of miR675 targeting for HDAC5. b The analysis of HDAC5 3′UTR luciferase reporter activity. c RT-PCR analysis for HDAC5 and Western blotting with anti-HDAC5. d Western blotting with anti-HDAC5. β-actin was used as internal control. e CHIP assay with anti-HDAC5 followed by PCR with Sirt1 promoter primers. f The analysis of Sirt1 promoter luciferase reporter activity. g The analysis of Sirt1 promoter luciferase reporter activity. h RT-PCR with Sirt1 cDNA primers. β-actin was used as internal control. i Western blotting with anti-Sirt1. β-actin was used as internal control. j Western blotting with anti-Sirt1 in the pCMV6-A-GFP group, pCMV6-A-GFP-HULC group, and pCMV6-A-GFP-HULC+rLV-HDAC5 group. β-actin was used as internal control