Fig. 5

HULC triggers the autophagy through Sirt1. a Co-IP with anti-LC3 followed by Western blotting with anti-Sirt1. b Co-IP with anti-Ac followed by Western blotting with anti-LC3. c Co-IP with anti-LC3 followed by Western blotting with anti-DOR. d Co-IP with anti-LC3 followed by Western blotting with anti-ATG4. e Co-IP with anti-ATG3 followed by Western blotting with anti-LC3 and ATG7. IgG IP served as negative control. f Western blotting with anti-Sirt1 and anti-LC3, respectively. g Western blotting with anti-Beclin-1 in four hLCSC lines. h The observation for autophagy (LC3-RFP) in hLCSCs. i The autophagy rate