Fig. 4

Enrichment of H3K27ac and binding of TFs in the enhancer sites. a Quantitative real-time PCR analysis of mRNA levels of the IFITM family after treatment with a low or a high LPS concentration. Gene expression was normalized to GAPDH transcript levels. The data represent three independent experiments. **P < 0.005. b Profile of H3K27 enrichment along the IFITM locus. Blue boxes indicate each gene; red boxes indicate enhancer regions. Upstream (R1, R2, R3) and downstream (R4, R5) enhancer regions of the IFITM1 gene are numbered. Orange boxes show the NF-κB binding sites; green boxes show the IRF1 binding sites (modified data from the TF search analysis of the UCSC Genome Browser using published ChIP-seq data from ENCODE). c Enrichment of H3K27ac on IFITM1 enhancer regions using ChIP-PCR. Enrichment was calculated relative to control input DNA from three independent experiments. *P < 0.05, **P < 0.005. d Enrichment of NF-κB on IFITM1 enhancer regions using ChIP-PCR. e Enrichment of IRF1 on IFITM1 enhancer regions using ChIP-PCR. d, e Enrichment was calculated relative to control input DNA from three independent experiments. **P < 0.005