Table 1 Media formulation

MET induction medium (M1)

MesenPRO-RS™

(ThermoFisher)

Vaporic acid (VPA, 500 μM; Sigma-Aldrich),

CHIR99021 (3 μM; Stemgent),

E-616452 (RepSox, 1 μM; Millipore), Tranylcypromine (5 μM; Tocris),

A-83-01 (500 nM; Tocris)

All-trans retinoic acid (atRA, 10 μM; Sigma-Aldrich)

Serum (2%, supplied from MesenPro kit)

Antibiotics-antimycotic (Invitrogen)

MET induction medium (M2)

MesenPRO-RS™

Same as M1 with reduced VPA (50 μM) and CHIR99021 (300 nM)

M2/CNT-50 medium

M2 and CNT-50 medium (CELLnTEC, Bern, Switzerland) (1:1 v/v)