Fig. 4

EphrinB2 overexpression promoted osteogenic differentiation of hDPSCs partially via enhancement of ephrinB2-mediated reverse signaling. a Overexpression of ephrinB2 elevated p-ephrinB2 but did not affect p-EphB4 and RhoA activity in hDPSCs during osteogenic differentiation. b Western blot analysis confirmed that ephrinB2 reverse signaling was activated by 1 μg/ml EphB4-Fc. c Alizarin Red S staining of hDPSCs treated with EphB4-Fc at different concentrations (0, 2, and 4 μg/ml) in osteogenic medium for 14 days. Scale bar = 200 μm. d Transcription of RUNX2, BMP2, and OCN in EphB4-Fc-treated hDPSCs on day 7 of osteogenic induction. Data are shown as mean ± SD. Assays were repeated three times. *p < 0.05 and **p < 0.01