Fig. 3

LGSC differentiation into acinar and ductal-like cells in vitro. a Immunofluorescent staining of LGCSs cultured for 14 days in 1/3 Matrigel. Most of the inner layer cells express Krt19 (red) and the outer layer cells express Krt14 (red); all cells express AQP5 (red); scale bar, 100 μm. Nuclear staining, DAPI (blue). b Immunofluorescent staining of mouse LG. Suprabasal cells located in inner layers of duct express Krt19 (red) and basal cells located in outer layers of duct express Krt14 (red); all secretory cells highly express AQP5 (red); scale bar, 100 μm. Nuclear staining, DAPI (blue). c The morphology of the LGSC culture for 14 days with addition of FBS; scale bar, 100 μm. d H&E staining of the LGSC culture for 14 days with addition of FBS. Acinar-like cells emerge in the LGSC sphere; scale bar,100 μm. e Immunofluorescent staining of the LGSC culture for 14 days with addition of FBS. The acinar-like cells highly express AQP5 (green), and all cells express epithelial cell marker E-cadherin (red); scale bar, 100 μm. Nuclear staining, DAPI (blue)