Fig. 4

Mesenchymal stem cell (MSC) transplantation restores PMFBP1 and HMGCL expression as well as polyamine content and cholesterol levels in double-toxin-induced MSA rats. Two-dimensional electrophoresis (2-DE) protein patterns in the brain tissues of 3 experimental groups. The brain striatum proteins were examined using 2-DE (a). Gel enlargement images (b) and relative expression levels of PMFBP1 and HMGCL (c) showing differential upregulation between the striatum extracts from 3 experimental group. The expressions of PMFBP1 and HMGCL were examined using specific antibodies in the brain striatum and substantia nigra (d). The polyamine levels were measured via high-pressure liquid chromatography (e), and the cholesterol levels were measured using CBC tests (f). *P < 0.05, significant difference vs. the saline-injected group. #P < 0.05, significant difference among the double-toxin-injected group. $ < 0.05, significant difference between two numbers of the MSC-injected group