Fig. 3

Cell populations and GO enrichment analysis of sorted chondroprogenitor cells. a scRNA-seq identified PDGFRβ⁺/CD146⁺/CD166⁺ cells contained at least 6 populations. b Expression of signature genes of each cell lineage. The sorted cells were enriched for mesenchymal and chondrogenic genes. c Percentage of total sorted chondroprogenitor cells in each unique cell population. Twenty-seven percent of the sorted were SOX9/COL2A1. Interestingly, a small percentage of cells (4% of total sorted cells) expressing SOX2 and NES was still observed. d PDGFRβ⁺/CD146⁺/CD166⁺ sorted cells may belong to the mesenchymal population (brown dashed circle) in unsorted cells. The green dashed circle indicates the population that was positive for SOX9 and COL2A1.e GO terms analysis (biological process) showing skeletal system development was highlighted in SOX9/COL2A1+ cells, while HMGB2/TOP2A+ and LGALS1/PTTG1+ cells were enriched in gene sets of cell division. See also Figure S1