Fig. 3

Determination of molecular mechanism in coelomic epithelium development stage. a A schematic diagram of gonadogenesis in 9.5–12 dpc. b Optical and IF micrographs were taken in different test groups at 12 days. DAPI staining showed blue in cell nucleus. EMX2⁺ cells showed green. Group (Induced IM) was the cells of differentiation model without genetic modification. Group (W) was the cells of differentiation model with overexpression of Wt1. Group (W.G.S1) was the cells of differentiation model with overexpression of Wt1, Gata4, and Sf1. The rest of the groups in this paper were done in the same manner. Scale bar = 200 μm. The transcriptional levels of cEmx2, dAmh, and eLhx9 were determined in different groups. Results were expressed relative to the highest mean value as mean ± SD (n = 3 independent experiments). Asterisks indicate statistical significance of differences in the mean gene expression calculated by one-way ANOVA method. (*P value < 0.05, **P value < 0.01, ***P value < 0.001). The transcriptional levels in different group when fWt1 was overexpressed or knock out (KO), hGata4 was overexpressed or KO, and jSf1 was overexpressed or KO were determined by qPCR at 12 days. Results were expressed related to the mean value of control group (group (Induced IM)) as mean ± SD (n = 3 independent experiments). The transcriptional levels when gWt1 was overexpressed, iGata4 was overexpressed, and kSf1 was overexpressed were determined by qPCR at 12 days. Results were expressed related to the mean value of control group (group (Induced IM)) as mean ± SD (n = 3 independent experiments). In i and k, nucleotide band of Dhh and Sry was detected. The band of the control group was on the left. The band of test group was on the right. l Cell wound scratch assay. Optical micrographs showed the cells right after scratch on the left, and the cells at the second day on the right. Scale bar = 200 μm