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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Transforming growth factor β1 accelerates and enhances in vitro red blood cell formation from hematopoietic stem cells by stimulating mitophagy

Fig. 2

TGF-β1 enhances maturation and enucleation of RBCs. a Representative flow panels showing expression of glycophorin A(CD235a) and transferrin receptor (CD71) during erythropoiesis in cultures set with and without TGF-β1. b Graph showing percentage of mature RBCs (CD235a+CD71−) in cell cultures with TGF-β1, as compared to control. Data show mean ± SEM from independent experiments done with cells from seven different donors. **p < 0.01, NS = non-significant. c Flow cytometric analysis showing enucleated RBCs. Enucleated RBCs percent is calculated as the percent of SYTO16− cells in the total cells excluding debris and nuclei. d Graph showing the percentage of enucleated RBCs in TGF-β1 set, compared to the control set at different time points. Results are presented as mean ± SEM from independent experiments done with five different donor samples. **p < 0.01, NS = non-significant

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Stem Cell Research & Therapy

ISSN: 1757-6512