Fig. 5

The chondrogenic differentiation of iPSCs with different concentrations of L2C4S4/TCP extracts in 14 days. a Immunofluorescence of chondrocytes specified proteins COL II, Aggrecan, and SOX9. b The average fluorescence intensity of COL II, Aggrecan and SOX9 proteins in each group, n = 5. c RT-PCR of chondrogenic genes Col2a1, Aggrecan, and Sox9, n = 3. Data presented as mean ± SEM. Scale bar = 200 μm. CTR: MCDM without any extracts. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, *p < 0.05, **p < 0.01, ***p< 0.001