Fig. 2
Mechanical load-induced expression of M1/M2-associated markers depends on H2S. a Representative immunohistochemical images of the compression side of distal roots. Expressions of M1-associated cytokines TNF-α and IFN-γ were upregulated after force was applied; the expressions decreased after HA injection and further enhanced after GYY4137 injection. Conversely, the expressions of M2-associated markers IL-10 and CD206 were hardly changed. Large boxed areas show high-magnification views of the small boxed areas. Arrow represents the direction of mechanical force. Scale bars: 100 μm. b Semiquantification of positive cells. N = 5–6; positive staining cells were counted on five different slides from each sample. The final result came from the average of three tests. **P < 0.01, ***P < 0.001 versus control. ###P < 0.001 versus force. &&&P < 0.001 versus force + HA