Fig. 3

EVs play a pivotal role in CM-mediated angiogenesis. a The proliferation of ECs exposed to H-CM, H-GW, P-CM, and P-GW was tested by CCK-8 assay (n = 3). b The migration of ECs stimulated by H-CM, H-GW, P-CM, and P-GW was detected by transwell assay (scale bar, 100 μm). c Quantitative analysis of the migrated cells in b (n = 3). d Representative images of the scratch wound assay of ECs treated with H-CM, H-GW, P-CM, and P-GW (scale bar, 200 μm). e Quantitative analysis of the migration rates in d (n = 3). f Representative images of tube formation in ECs treated with H-CM, H-GW, P-CM, and P-GW (scale bar, 200 μm). g Quantitative analyses of the covered area, total branching points, total loops, and total tube length in f (n = 3). *P < 0.05, **P < 0.01 vs. the H-CM group; ^#P < 0.05, ^##P < 0.01 vs. the P-CM group