Fig. 7
HucMSC-Ex mediates PARP-1 and AIF signaling to inhibit H2O2-induced cell death. a Western blot of nuclear AIF and PARP-1 and mitochondrial AIF expression in HaCaT cells treated with hucMSC-CM, hucMSC-dp-Ex, or hucMSC-Ex for 24 h after H2O2 induction. b Mitochondrial AIF in the hucMSC-dp-Ex-treated HaCaT cells decreased while that for the hucMSC-ex-treated HaCaT cells increased (n = 3; **P < 0.01). Nuclear AIF and PARP in the hucMSC-dp-Ex-treated HaCaT cells were higher than those in the hucMSC-ex-treated HaCaT cells (n = 3; **P < 0.01). PARP activation in response to the addition of various culture media to the HaCaT cells as detected by western blot using anti-PAR. c, d PAR in hucMSC-Ex-treated HaCaT cells decreased compared to that for the other treatments (n = 3; ***P < 0.001). e–g Representative immunofluorescence images and (h) quantitative analyses of TUNEL (green), AIF (red), and PAR (green) expressed in HaCaT cells after the addition of various culture media. Blue indicates the nucleus and pink indicates AIF translocation into the nucleus. Scale bar = 200 μm. Compared to control: *P < 0.05 and **P < 0.01; compared to H2O2:#P < 0.05 and ##P < 0.01. Data are representative of three independent experiments (means ± SD)