Fig. 3

DNA methylation patterns do not clearly reflect subclones in iMSCs. a Bisulfite barcoded amplicon sequencing reveals changes in mean DNA methylation levels during expansion of iMSCs in GRM7, CASR, and PDE4C (representative CpG sites are depicted).b Frequencies of different DNA methylation patterns in individual reads of the amplicons of GRM7, CASR, and PDE4C of iMSCs at early versus late passages (red = methylated; blue = non-methylated). The height is indicative for the frequency of the corresponding pattern. P, passage. c Changes in the composition of different DNA methylation patterns during culture of iMSCs