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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Analysis of multi-lineage gene expression dynamics during primordial germ cell induction from human induced pluripotent stem cells

Fig. 3

Efficiency of PGCLC induction from iPSCs in vitro. a FACS analysis by EpCAM and INTEGRINα6 expression of cells during hPGCLC induction (until day 8) from hiPSCs by BMP4, LIF, SCF, and EGF (left) or by no cytokines (right). Boxed areas indicate EpCAM/INTEGRINα6 double-positive cells with their percentages. b Percentage of cells double positive for EpCAM/INTEGRINα6, only positive for EpCAM and only positive for INTEGRINα6 in day 2, 4, 6 and 8 floating embryoids determined by FACS, respectively. Error bars indicate mean ± SD of three independent experiments. *p < 0.001 vs. the day 2 groups

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Stem Cell Research & Therapy

ISSN: 1757-6512