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Fig. 10 | Stem Cell Research & Therapy

Fig. 10

From: Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

Fig. 10

The potential relationship between G043225, miR-588, and FBN1. a, b Dual-luciferase reporter assay was used to detect the binding sites between G043225 and miR-588, and FBN1 and miR-588. Ectopic expression of miR-588 led to a significant decrease of the reporter luciferase activity with the WT (wild type) but not that of the MUT (mutant) reporter. c The increased expression level of miR-588 was determined following interference of G043225 at 14 days after the odontogenic induction by qRT-PCR. d, e The decreased mRNA and protein expression level of FBN1 was determined in hDPSCs following interference of G043225 at 14 days after the odontogenic induction by qRT-PCR and western blot. GAPDH was used as an internal control. Data represent the mean ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with differentiated shRNA-ctrl group

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Stem Cell Research & Therapy

ISSN: 1757-6512