Fig. 3

Freshly harvested or cryopreserved CD362⁺ UC-hMSCs have comparable therapeutic effects in E. coli-induced lung injury. Freshly harvested from culture or thawed from cryostorage and immediately administered, CD362⁺ UC-hMSCs ameliorated E. coli ARDS-induced decrement in arterial oxygenation (a). There were no significant changes in lung tissue wet to dry ratio (b). Freshly harvested CD362⁺ UC-hMSCs alone significantly reversed the decrease in lung static compliance (c). Both fresh and cryopreserved CD362⁺ UC-hMSCs reduced lung E. coli bacterial load (d), total BAL infiltrating leukocytes (e) BAL neutrophils (f), BAL IL-1β (g), and BAL IL-6 (i), and had a trending but not significant effect on CINC-1 (h) in this series. Abbreviations: vehicle, treatment with vehicle alone; UC 362⁺ fresh, umbilical cord-derived CD362⁺ hMSC freshly harvested from culture; UC 362⁺ cryo, umbilical cord-derived CD362⁺ hMSC thawed from cryostorage and immediately administered; BAL, bronchoalveolar lavage; IL-1β, interleukin 1 beta; CINC-1, cytokine-induced neutrophil chemoattractant 1; IL-6, interleukin 6. Error bars represent standard deviation. *Significantly (P < 0.05) different from vehicle control group