Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells

Table 1 Primary antibodies

Antibody	Source	Reference	Type	Use	Dilution
Aquaporin-4	Sigma-Aldrich	A5971	RbP	FR	1:200
BDNF	Abcam	ab108319	RbM	I, VB	1:250
CD11b	BioLegend	101225	RtM	FC	1:80
CD34	BioLegend	119309	MM	FC	1:25
CD44	BioLegend	103015	MM	FC	1:50
CD45.1	BioLegend	110713	MM	FC	1:80
CD73	BD	561543	RtM	FC	1:25
CD90.2	BioLegend	105315	RtM	FC	1:200
Collagen type II	Calbiochem-Novabiochem	AB746P	RbP	C	1:40
F4/80	Biolegend	123107	RtM	I, FC	1:200
GAPDH	Abcam	Ab9485	RbP	WB	1:2500
GDNF	Santa Cruz	sc-328	RbP	I, FC, VB	1:100
GFAP	Sigma	G-A-5	MM	I, FC, VB, WB	1:1000
δGFAP	Merck	AB9598	RbP	I	1:500
Glutamine synthetase	Abcam	Ab49873	RbP	VB	1:5000
Iba1	Wako	019-19741	RbP	FR, VB, WB	1:500, 1:1000 (WB)
Myeloperoxidase	Abcam	Ab139748	RbP	VB	1:100
Nestin	BIO-RAD	AHP1739	RbP	I, FC, VB	1:100
NeuN	Merck	MAB377	MM	I, FC, VB	1:100
NG2	Merck	AB5320	RbP	I	1:200
NGF	Abcam	AB6199	RbP	I, VB	1:500
RFP	Chromotek	5F8	RtM	FC, VB	1:500
β-III tubulin	Promega	A6712	MM	I, FC	1:5000
VEGF	Abcam	Ab46154	RbP	I, FC, VB	1:1000

Abbreviations: C BM-MSC in cell cultures, I immunofluorescence for BM-MSC on slides, FC flow citometry, FR frozen sections, MM mouse monoclonal, RbM rabbit monoclonal, RtM rat monoclonal, RP rabbit polyclonal, VB vibratome sections, WB western blot. Sources: AbCam, Cambridge, UK; BD, San Diego, CA, USA; BioLegend, San Diego, CA, USA; BIO-RAD, Oxford, UK; Chromotek, Planegg-Martinsried, Germany; GeneTex, Irvine, CA, USA; Merck Millipore, Burlington, MA, USA; Promega, Madison, WI, USA; Santa Cruz Biotechnology, Dallas, TX, USA; Sigma-Aldrich

ISSN: 1757-6512