Fig. 4

The RNA profile comparison between the human MSCs cultured with NBVbe or 5% PL. a Venn diagram showing the numbers of genes differentially regulated in human MSCs cultured with NBVbe, 1% PL, 2%PL, or 5% PL medium. b Hierarchical clustering analysis of mRNA profiles in human MSCs cultured with NBVbe, 1% PL, 2%PL, or 5% PL medium. c Differentially expressed genes between the human MSCs cultured with NBVbe and 5%PL. d KEGG pathway enrichment for differentially expressed genes between the human MSCs cultured with NBVbe and 5% PL. e Alternative splicing events detected by RNA sequencing. f Alternative splicing events compared with the human MSCs cultured with DMEM via RNA sequencing analysis. g SNV (single-nucleotide variant) and InDel (insertion and deletion mutations) detected by DNA exon sequencing. NC, negative control, human MSCs cultured with DMEM; DEGs, differentially expressed genes; A5SS, alternative 5′ splicing site; A3SS, alternative 3′ splicing site; MXE, mutually exclusive exons; RI, retained intron; SE, skipped exon