Fig. 5

The NBVbe medium supports the human MSC isolation from the umbilical cords. a Cell isolation reagents comparison. Cell number was counted 30 days after the cell isolation. b Human MSCs were isolated with the different concentration of collagenase B. Cell number was counted 30 days after the cell isolation. c Human MSCs isolated from the umbilical cord, passage 0 and 6 cultured with the NBVbe medium. d Flow cytometry analysis of the human MSCs after isolated and cultured with the NBVbe medium. e Human MSCs were isolated and expanded with the NBVbe medium and then differentiated into adipocytes, osteocytes, and chondrocytes. Left: Oil Red O staining and anti- FABP4 immunostaining for the differentiated adipocytes; middle: Alizarin Red S staining and anti-Osteocalcin immunostaining for the differentiated osteocytes; right: Alcian blue staining and anti-Aggrecan immunostaining for the differentiated chondrocytes. Scale bar, 200 μm. An asterisk indicates P < 0.05. GCD, gentle cell dissociation reagent; SR, ReLeSR™; CDB, cell dissociation buffer, enzyme-free; ACF, animal component-free cell dissociation kit