Fig. 2

Cholesterol induces apoptosis in TDSCs. a, b Cells were treated with 10 mg/dL cholesterol for 24 h and analyzed using annexin V-FITC flow cytometry. c, d DNA fragmentation was detected by TUNEL staining and observed by fluorescence microscopy. e, f Cells were incubated with cholesterol (10 mg/dL) for different times. The apoptosis-related proteins cleaved caspase-3, Bax, Bcl-xL, and PARP were analyzed by western blotting. All quantitative data are expressed as the means ± SEM of the results from three independent experiments. *p < 0.05 versus control. CHO, cholesterol