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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Loss of receptor tyrosine kinase-like orphan receptor 2 impairs the osteogenesis of mBMSCs by inhibiting signal transducer and activator of transcription 3

Fig. 5

In Ror2-knockdown mBMSCs, the expression of Stat3 was decreased. ac Quantitative real-time PCR (a) and western blot (b, c) analysis of the expression of Stat3 after osteogenic induction for 3 weeks. n = 3, *P < 0.05 vs. mBMSC-mock1. d Immunocytofluorescence staining showed the distribution of Stat3 after osteogenic induction for 24 h. Scale bar = 50 μm. e Western blot analysis revealed the expression of nuclear Stat3 and pStat3 after osteogenic induction for 24 h. f, g Overexpression of Stat3 in Ror2-knockdown mBMSCs. Stat3 protein expression was evaluated using western blot analysis. n = 3, $P < 0.05 vs. mBMSC-sh-Ror2

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