Fig. 1

A schematic diagram of Epi-SCs and SKP transplantation for hair follicle regeneration. Dorsal skin tissue in full thickness was collected from neonatal C57 mice (a), which was cut into pieces (b). The tissue was separated into the epidermis and dermis after treatment with dispase II (c, d). Epidermal stem cells (Epi-SCs) were derived from the epidermis as described in the “Methods” section, which grew in monolayer (e) and expressed CD49f as detected by immunofluorescence staining (red, g). Skin-derived precursors (SKPs) were derived from the dermis as described in the “Methods” section, which were grown in spheroids and expressed nestin as detected by immunofluorescence staining (red, h). Full thickness excisional skin wounds were prepared in nu/nu mice (i), and a mixture of Epi-SCs and SKPs in Matrigel was implanted into the wound, which resulted in the growth of black hairs (j). Scale bar, 50 μm