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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: HIF1α overexpression enhances diabetic wound closure in high glucose and low oxygen conditions by promoting adipose-derived stem cell paracrine function and survival

Fig. 4

HIF1α overexpression restores ADSC survival under high glucose and low oxygen. a Intracellular ROS levels in control and HIF1α-overexpressing ADSCs cultured in high glucose were determined using DCFH-DA. b 8-OHdG levels were evaluated by ELISA. c The proliferation of control and HIF1α-overexpressing ADSCs under high glucose was analyzed by MTT assay. d Apoptosis in control and HIF1α-overexpressing ADSCs cultured in high glucose was analyzed using Annexin V-FITC/PI staining and flow cytometry. e Cell cycle distributions of ADSCs in each group. All experiments were performed under hypoxia. **p < 0.01; *p < 0.05; Control, normoglycemia; High, high glucose; HIF1α, HIF1α overexpression

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Stem Cell Research & Therapy

ISSN: 1757-6512