Fig. 5

Adipogenic potential of the muscle- and bone-derived MSCs. Representative images (a) and quantification (b) from Oil Red O staining analysis for the muscle- and bone-derived MSCs. Oil Red O-positive adipocytes were counted (b, left) and expression of adipogenic genes was measured to assess adipogenesis (b, right). Bone-derived MSCs from patients with OA showed lower adipogenic potential compared to those from OP patients (0.242 and 8.726%, p = 0.031) (b, left). No significant differences were observed for the muscle-derived MSCs. No significant differences were observed for the expression of the adipogenic genes (b, right). Scale bars: 200 μm. PPARG, peroxisome proliferator-activated receptor-gamma; ADIPOQ, adiponectin; FABP4, fatty acid-binding protein 4. Scatter plots showing the correlation between CD271 gene expression and proportions of Oil Red O-positive adipocytes in muscle-derived MSCs (left, Spearman correlation), and no correlations for bone-derived MSCs (right) (c)