Inflammatory priming enhances mesenchymal stromal cell secretome potential as a clinical product for regenerative medicine approaches through secreted factors and EV-miRNAs: the example of joint disease

Table 1 EV-miRNAs in the first quartile of expression that are involved in cartilage-protective/degenerative mechanisms

	% weight EVs/iEVs	Down IFNγ	Up IFNγ	Only EVs	Target genes	Functions
Cartilage protective
hsa-miR-21-5p	5.13/6.73				GAS5, GDF5	Autophagy
hsa-miR-222-3p	5.74/7.61				MMP13, HDAC4	Cartilage degradation
hsa-miR-138-5p	0.19/0.21				SP1, HIF-2Α	Chondrocyte differentiation
hsa-miR-24-3p	19.63/18.47				P16INK4A	Chondrocyte differentiation and apoptosis
hsa-miR-210-3p	0.23/0.56		X		DR6, NF-ΚB	Inflammation, chondrocyte apoptosis
hsa-miR-26a-5p	0.64/1.06				NF-ΚB, CD200, COL10A1, COL9A1, CTGF	Inflammation, modulate ECM homeostasis
hsa-miR-130a-3p	0.57/0.78				TNF-Α	Inflammation
hsa-miR-149-5p	0.13/0.02	X			TNFΑ, IL-1, IL-6	Inflammation
hsa-miR-199a-3p	0.92/0.97				COX-2	Inflammation
hsa-miR-320-3p	0.00/0.53			X	MMP13	Matrix degradation
Cartilage destructive
hsa-miR-21-5p	5.13/6.73				GDF5	Chondrocyte differentiation and homeostasis
hsa-miR-145-5p	1.84/0.79	X			SOX9, SMAD3	Chondrocyte differentiation and homeostasis
hsa-miR-16-5p	0.40/0.44				SMAD3	Chondrocyte differentiation and homeostasis
hsa-miR-193b-5p	5.00/5.56				TGF-Β2, TGF-ΒR3, SOX9, COL2	Chondrocyte differentiation and homeostasis
hsa-miR-29b-3p	0.07/0.27				SMAD, NF-ΚB, WNT	Chondrocyte differentiation and homeostasis
hsa-miR-34a-5p	0.70/0.93				COL2Α1, INOS	Chondrocyte apoptosis
hsa-miR-483-5p	0.12/0.11				BMP7, TGFΒ, IL-1Β, MMP13	Inflammation

ISSN: 1757-6512