Fig. 6

AD-MSC-conditioned medium administration reduces sciatic nerve inflammation in diabetic mice. a, b Representative confocal images of longitudinal sections of sciatic nerves obtained from 26-week-old non-diabetic and diabetic mice treated with vehicle, conditioned medium derived from non-preconditioned AD-MSCs, or conditioned medium derived from DFX-preconditioned AD-MSCs. T lymphocytes were detected with anti-CD3 antibody (a) while macrophages were detected with anti-CD11b antibody (b). Nuclei were stained with DAPI. Arrows indicates CD3⁺ cells or CD11b⁺ cells. c Quantification of CD3⁺ cells in sciatic nerve. Data are presented as number of CD3⁺ cells per 0.5 mm² of sciatic nerve. d Quantification of CD11b⁺ cells in sciatic nerve. Data are presented as number of CD11b⁺ cells per 0.5 mm² of sciatic nerve. e Quantification of mRNA levels of the pro-inflammatory cytokines IL-1β and TNF-α in the sciatic nerve. Data were normalized against the housekeeping gene β-actin. Quantitative data are presented as mean ± S.E.M. (n = 6, one-way ANOVA with Tukey post-test, *p < 0.05)