Fig. 6

Effect of NF-κβ pathway inhibition on VTN expression, cell cycle status, and cell viability. a WJ-MSCs were transfected with NC siRNA or p65 esiRNA and gene expressions of VTN, CCNA2, E2FA, and AURKA was examined using qRT-PCR (n = 3). b Protein expression of p65 was confirmed to be lowered in the p65 specific knocked down samples by immunoblotting. Band densities were quantified and plotted relative to GAPDH, which was used as a loading control. Blots are representative of three independent biological samples (n = 3). c Cell cycle phase analysis by flow cytometry. Percentages of cells in each phase of the cell cycle are depicted in the histogram. Bar represents mean ± SEM (n = 4). d The percentages of viable, early apoptotic, and late apoptotic population of WJ-MSCs transfected with NC siRNA or p65 esiRNA under serum deprivation state are compared and demonstrated using bar graphs. Representative data of apoptosis analysis using flow cytometry are shown. Each bar represents mean ± SEM (n=3). Serum-deprived condition represented as no serum (NS) in the figure (*p < 0.05, ***p < 0.0001)