Table 1 Primer sequences used for quantitative RT- PCR
Gene | Accession number | Forward primers (5′-3′) | Reverse primers (5′-3′) | Amplicon size (bp) |
|---|---|---|---|---|
GAPDH | NM_002046.7 | GAGTCAACGGATTTGGTCGT | TTGATTTTGGAGGGATCTCG | 238 |
AURKA | NM_198433.3 | CATGATGCTACCAGAGTCTACC | GAGATCCACCTTCTCATCATGC | 336 |
CCNA2 | NM_001237.5 | CTGCATTTGGCTGTGAACTAC | ACAAACTCTGCTACTTCTGGG | 143 |
CDKN3 | NM_005192.4 | GGCAATACAGACCATCAAGCAA | TGATGATAGATGTGCAGCTAATTTGT | 73 |
EF2A | NM_005225.3 | CATCCAGCTCATTGCCAAGAAG | GATCCCACCTACGGTCTCCTCA | 391 |
p21 | NM_000389.5 | GAGGCCGGGATGAGTTGGGAGGAG | CAGCCGGCGTTTGGAGTGGTAGAA | 221 |
p27 | NM_004064.4 | CCGGCTAACTCTGAGGACAC | AGAAGAATCGTCGGTTGCAG | 120 |
p53 | NM_000546.5 | GAGCTGAATGAGGCCTTGGA | CTGAGTCAGGCCCTTCTGTCTT | 151 |
VTN | NM_000638.4 | GGGTCTACTTCTTCAAGGGGAA | AATGAACTGGGGCTGTCTGG | 197 |