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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Bone marrow-derived from the human femoral shaft as a new source of mesenchymal stem/stromal cells: an alternative cell material for banking and clinical transplantation

Fig. 1

Primary culture of iliac crest BM-MSC and cell morphological characterization at different culture stages—a the comparison of three different isolations protocols. b Visualization of bone marrow-derived cells cultured at initial passage 0 and after 14 DIV. c The number of mononuclear cell concentration obtained after different gradient centrifugations. (F) Ficoll, (S) 17.5% sucrose; the whole fraction of BM and the total cell count for MSC. d Graphs show population doubling time (PDT) of BM-MSC in commercial medium 1 versus medium 2. Scale bars = 200 μm. All data reflect mean ± SD from ≥ 5 independent experiments

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Stem Cell Research & Therapy

ISSN: 1757-6512