Fig. 4

H19 binds directly to miR-140-5p and overexpression of miR-140-5p inhibits odontoblastic differentiation of hDPSCs. a Databases consistently predicted six miRNAs interacting with lncRNA H19. b Relative candidate miRNA expression after odontoblast induction for 14 days compared with that in NC groups. c Sequence alignment of H19 with wild-type and mutant-type miR-140-5p. The potential binding sites were predicted by Starbase. d Relative luciferase activity of HEK293T cells co-transfected with H19 reporter plasmid and miR-140-5p mimics. e qRT-PCR analysis was performed to measure the levels of miR-140-5p. f After odontoblast induction for 7 days, relative mRNA expressions of ALP, RUNX2, DSPP, and DMP-1 in miR-140-5p mimic group were measured by qRT-PCR. g Relative mRNA expressions of ALP, RUNX2, DSPP, and DMP-1 in miR-140-5p mimic groups and miR-140-5p mimic groups after odontoblast induction for 14 days. h Relative protein expression of RUNX2, DSPP, and DMP-1 in miR-140-5p mimic transfection groups after odontoblast induction for 7 days and 14 days. i Representative micrographs of Alizarin Red S staining in miR-140-5p mimic transfection groups after odontoblast induction for 7 days and 14 days. The data are presented as the mean ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001