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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Generation of gene-corrected functional osteoclasts from osteopetrotic induced pluripotent stem cells

Fig. 1

Lentiviral-mediated gene expression in IMO iPSC-derived monocytes. Schematic representation of the experimental design (a). IMO iPSCs were transduced with a lentiviral vector expressing TCIRG1 and containing the CBX3-UCOE element (b). EBs were generated from the iPSCs, transferred to tissue culture plates after 4 days of culture, and differentiated in X-VIVO media supplemented with M-CSF and IL-3. The presence of GFP+ cells was verified throughout the culture at iPSC stage (c, d), at EB stage (e), and during EB differentiation (f)

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Stem Cell Research & Therapy

ISSN: 1757-6512