Table 1 Primer list [18, 19]
Target | Product size (bp) | Primer sequence |
|---|---|---|
Sendai vector detection | ||
SeV | 181 | Forward GGATCACTAGGTGATATCGAGC |
Reverse ACCAGACAAGAGTTTAAGAGATATGTATC | ||
KLF4 | 410 | Forward TTCCTGCATGCCAGAGGAGCCC |
Reverse AATGTATCGAAGGTGCTCAA | ||
CMYC | 532 | Forward TAACTGACTAGCAGGCTTGTCG |
Reverse TCCACATACAGTCCTGGATGATGATG | ||
SOX2 | 451 | Forward ATGCACCGCTACGACGTGAGCGC |
Reverse AATGTATCGAAGGTGCTCAA | ||
OCT4 | 483 | Forward CCCGAAAGAGAAAGCGAACCAG |
Reverse AATGTATCGAAGGTGCTCAA | ||
Pluripotency and differentiation markers | ||
NANOG | 237 | Forward AAGGTCCCGGTCAAGAAACAG |
Reverse CTTCTGCGTCACACCATTGC | ||
BRACHYUR | 252 | Forward TAAGGTGGATCTTCAGGTAGC |
Reverse CATCTCATTGGTGAGCTCCCT | ||
GOOSECOID | 89 | Forward AACGCGGAGAAGTGGAACAAG |
Reverse CTGTCCGAGTCCAAATCGC | ||
MIXL1 | 67 | Forward CTGTTCCCCTCTCTCTGAAGA |
Reverse GGCAGAAAAGATGTGTTCCTCC | ||
FOXA2 | 83 | Forward GGAGCAGCTACTATGCAGAGC |
Reverse CGTGTTCATGCCGTTCATCC | ||
SOX17 | 94 | Forward GTGGACCGCACGGAATTTG |
Reverse GGAGATTCACACCGGAGTCA | ||
NESTIN | 145 | Forward TTGCCTGCTACCCTTGAGAC |
Reverse GGGCTCTGATCTCTGCATCTAC | ||
SOX1 | 287 | Forward CAGTACAGCCCCATCTCCAAC |
Reverse GCGGGCAAGTACATGCTGA | ||
NEUROD | 523 | Forward GCCCCAGGGTTATGAGACTATCACT |
Reverse CCGACAGAGCCCAGATGTAGTTCTT | ||
OTX2 | 98 | Forward TGTAGAAGCTATTTTTGTGGGTGA |
Reverse GAGCATCGTTCCATCTAACTTTTT | ||
PAX6 | 162 | Forward TGTCCAACGGATGTGTGAGT |
Reverse TTTCCCAAGCAAAGATGGAC | ||
IMO mutation detection | ||
Human TCIRG1 Exons 16–20 | 1255 | Forward GGTTCCTTTGCAGGTGTGCA |
Reverse CCTCTCCTGCCTCAGAGGTC | ||