Fig. 1
CPCs senescence was associated with poor outcome of distraction arthroplasty in the treatment of OA. a Representative HE staining (left) and Saf-O staining (right) of the medial femoral condyles of one control and one FAIL patient. The AOFAS score of the control case as an example of the scoring results is 89, while the AOFAS score of the FAIL case is 24. b AOFAS scores of the patients (N = 6 donors per group). c Representative immunofluorescence staining for CD105 (green), P53 (red), and DAPI (blue) of the medial femoral condyles of the patients. d Quantitative analysis of the percentage of CD105+/P53+ cells (three random fields were selected, n = 6 sections/donor, N = 6 donors per group). e ELISA assay for the IL-1β level in the ankle joints of the patients (N = 6 donors per group). f Linear correlation analysis of the percentage of CD105+/P53+ cells and the AOFAS scores. R2 = 0.4223, P < 0.0001. g Linear correlation analysis of the IL-1β level and the AOFAS scores. R2 = 0.7677, P = 0.0002. a Scale bar 200 μm. c Scale bar 50 μm. b, d, e Values are shown as mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test