Fig. 7

Eliminating senescent CPCs by intra-articular injection of senolytics improved cartilage regeneration in an IHP model. The rats were divided into four groups randomly: one group left untreated (control group) and the other three groups treated by distraction arthroplasty alone (DIS group) or together with vehicle (DIS+DMSO group) or with DQ (DIS+DQ group) (N = 5 rats per group). a Representative macroscopic photos, micro-CT, histological staining, and immunohistochemical staining for Col 2 of the femoral condyles of the rats (n = 6 sections/rat, N = 5 rats per group). b, c Quantitative analysis of OARSI scores (b) and relative Col 2 levels (c). d–g Quantitative analyses of the micro-CT parameters of the femoral subchondral bone. h ELISA assay for the IL-1β level in the serum. a Scale bar 200 μm. b–h Values are shown as mean ± SD. NS, no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA with Tukey’s multiple comparisons test